Anti-Rat CINC-2 α/β

Anti-Rat CINC-2 α/β

Product No.: C1463


Target CINC-2 alpha / beta Product Type Polyclonal Antibody Alternate Names Cytokine-Induced Neutrophil Chemoattractant 2 Alpha/Beta Applications N , WB


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Antibody Details Product Details Reactive Species Rat Host Species Goat Immunogen E. coli - Derived Recombinant Rat CINC-2 α Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828527 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat CINC-2 α/β this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit forrrCINC-2α isapproximately 2 ng/lane and 20 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat CINC-2 α/β bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Rat CINC-2 α/β (at 0.05 ng/ml) is 0.5 - 2 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Goat Anti-Rat Cytokine-Induced Neutrophil Chemoattractant 2 Alpha/Beta (CINC-2 α/β) recognizes Rat CINC-2 α/β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. PubMed CINC-2 alpha / beta NCBI Gene Bank ID 171551 UniProt.org More Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Rat

Host Species

Goat

Immunogen

E. coli - Derived Recombinant Rat CINC-2 α

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828527

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Rat CINC-2 α/β this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit forrrCINC-2α isapproximately 2 ng/lane and 20 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Rat CINC-2 α/β bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Rat CINC-2 α/β (at 0.05 ng/ml) is 0.5 - 2 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Rat Cytokine-Induced Neutrophil Chemoattractant 2 Alpha/Beta (CINC-2 α/β) recognizes Rat CINC-2 α/β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

PubMed

CINC-2 alpha / beta

NCBI Gene Bank ID

171551

UniProt.org

More Information on Uniprot.org