Anti-Rat CNTF (Clone 34738)

Purified Recombinant Rat CNTF (>98%)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

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Neutralization: This antibody is useful for neutralization of Rat CNTF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Rat CNTF (at 200 ng/ml) is 1 - 3 µg/ml.

Mouse Anti-Rat Ciliary Neurotrophic Factor (CNTF) (Clone 34738) recognizes an epitope on Rat CNTF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Ciliary neurotrophic factor (CNTF) is a cytokine¹ that supports survival and/or differentiation of a variety of neuronal cell types including sensory, sympathetic, and motoneurons.² With these abilities, CNTF has implications for Parkinson's disease and cell-replacement therapies for other disorders.³ CNTF mimics the biological actions of leptin while overcoming "leptin resistance" and appears to reduce inflammatory signaling cascades associated with lipid accumulation in liver and skeletal muscle. The advantages and disadvantages of CNTF has shown promise as a therapeutic strategy to alleviate obesity-associated diseases.⁴

1. Boschero, AC. et al. (2009) Cytokine 46(1):65-71
2. Thoenen, H. et al. (1994) J Neurobiol. 25: 1436
3. Hagg, T. et al. (2008) J Neurosci. 28: 2231
4. Febbraio, MA. et al. (2008) J Mol Med. 86: 353