Anti-Rat CXCL2 (Clone 123802)

Product No.: C1433


Clone 123802 Target CXCL2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names SCYB2, GRO2, Growth-Regulated Protein Beta (GROb), MIP-2a, MGSA-b, CINC-2a, MIP-2, Cytokine-Induced Neutrophil Chemotactic Factor 3 (CINC-3), Chemokine (C-X-C Motif) Ligand 2 Isotype IgG2b Applications ELISA Cap , N , WB


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Antibody Details Product Details Reactive Species Rat Host Species Mouse Immunogen Purified Recombinant Rat CXCL2 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828494 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:C1509 at a concentration of approximately 50 ng/ml. A suggested standard for this assay is PN:C1343. Western Blotting: To detect Rat CXCL2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1343. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat CINC-3 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Rat CINC-3 (at 0.1 ng/ml) is 0.4 - 2 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Rat Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Clone 123802) recognizes an epitope on Rat CXCL2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Chemokine (C-X-C motif) ligand 2 (CXCL2) is an inducible murine chemokine involved in attraction of polymorphonuclear granulocytes to sites of infection.¹ This chemokine is secreted by monocytes and macrophages and is chemotactic for hematopoietic stem cells.^2,3,4 PubMed CXCL2 Research Area Chemokine References & Citations 1. Beuscher, HU. et al. (2004) International Immunol. 16: 1675 2. Cerami, A. et al. (1989) Proc Nat Acad Sci. 86: 612 3. Grotendorst, GR. et al. (1990) Mol Cell Biol. 10: 5596 4. Fukuda, S. et al. (2006) Exp Hematol. 34: 1010 View product citations for antibody C1433 on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Rat

Host Species

Mouse

Immunogen

Purified Recombinant Rat CXCL2 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828494

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:C1509 at a concentration of approximately 50 ng/ml. A suggested standard for this assay is PN:C1343.
Western Blotting: To detect Rat CXCL2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1343.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Rat CINC-3 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Rat CINC-3 (at 0.1 ng/ml) is 0.4 - 2 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Rat Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Clone 123802) recognizes an epitope on Rat CXCL2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Chemokine (C-X-C motif) ligand 2 (CXCL2) is an inducible murine chemokine involved in attraction of polymorphonuclear granulocytes to sites of infection.¹ This chemokine is secreted by monocytes and macrophages and is chemotactic for hematopoietic stem cells.^2,3,4

PubMed

CXCL2

Research Area

Chemokine

References & Citations

1. Beuscher, HU. et al. (2004) International Immunol. 16: 1675
2. Cerami, A. et al. (1989) Proc Nat Acad Sci. 86: 612
3. Grotendorst, GR. et al. (1990) Mol Cell Biol. 10: 5596
4. Fukuda, S. et al. (2006) Exp Hematol. 34: 1010

View product citations for antibody C1433 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
M1277	Anti-Mouse CXCL2 (Clone 40605)
G122	Anti-Rat CXCL2
C1433	Anti-Rat CXCL2 (Clone 123802)
C1465	Anti-Rat Chemokine Ligand 2
G706	Recombinant Human GROβ/CXCL2 (aa 39-107)
G131	Recombinant Human CXCL2
M178	Anti-Mouse CXCL2
C1509	Anti-Rat Chemokine (C-X-C Motif) Ligand 2 (CXCL2) – Biotin
M195	Anti-Mouse CXCL2-Biotin
C1609	Rat CINC-3/CXCL2 ELISA Development Kit

Formats Available

Prod No.	Description
C1433	Anti-Rat CXCL2 (Clone 123802)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Rat CXCL2 (Clone 123802)