Anti-Rat TNF-α (Clone 45418)
Anti-Rat TNF-α (Clone 45418)
Product No.: T442
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Clone 45418 Target TNF-α Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TNF-alpha, TNFSF2, Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin Isotype IgG1 Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen Purified Recombinant Rat TNF-α (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831942 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T189 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T157. Western Blotting: To detect Rat TNF-α this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat TNF-α is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T157. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat TNF-α bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat TNF-α (at 0.025 ng/ml) is 10 - 40 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 45418 recognizes an epitope on rat TNF-α. This antibody shows 100% cross-reactivity with rmTNF-α and no cross-reactivity with rfeTNF-α. Background TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Antigen Distribution TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells. PubMed References & Citations1. El-Harith, EHA. et al. (2004) Saudi Med J. 25: 135 2. Adolf, GR. et al. (1990) Infec Immun. 58: 3996 Technical Protocols N  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
