Anti-Respiratory Syncytial Virus (Clone: RSV-17E10) – Biotin
Anti-Respiratory Syncytial Virus (Clone: RSV-17E10) – Biotin
Product No.: R224
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Product No.R224 Clone RSV-17E10 Target RSV Product Type Recombinant Monoclonal Antibody Alternate Names RSV, Orthopneumovirus Isotype Human IgG1λ Applications ELISA , FA , N , WB |
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Antibody DetailsProduct DetailsReactive Species Respiratory Syncytial ⋅ Virus Expression Host HEK-293 Cells Immunogen Human donors targeting the post-fusion RSV F protein using human hybridoma technology Product Concentration 0.5 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC Formulation This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Standard Overnight on Blue Ice. Applications and Recommended Usage? Quality Tested by Leinco WB ELISA Additional Applications Reported In Literature ? FA N Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity RSV-17E10 activity is directed against antigenic site IV of the RSV and hMPV fusion (F) proteins and binds to pre- and post-fusion F proteins of RSV A strain A2 and RSV B strain 18537.
Antibody RSV-17E10 is also capable of neutralizing RSV A2, RSV Long viruses (subgroup A), RSV 18537 B, and RSV WV/401R viruses (subgroup B). Competition-binding assay shows that RSV-17E10 targets antigenic site IV. The mode of action is independent of R429. RSV-17E10 is cross-reactive with hMPV F at antigenic site IV and neutralizes hMPV better than RSV. Cross-neutralization of RSV and hMPV is associated with recognition of the G430 and I432 residues, which are shared between RSV and hMPV F proteins. RSV-17E10 binds at a unique ~56° tilt, which may be a determinant of cross-reactivity with hMPV F. Background Respiratory syncytial virus (RSV) is a common respiratory virus that infects the majority of children by two years old1, 2. While usually mild, RSV can be serious in infants and older adults and is the leading cause of bronchiolitis and pneumonia in children less than one year of age in the United States1. A related pneumovirus, human metapneumovirus (hMPV), also significantly contributes to hospitalizations resulting from lower respiratory tract infection2. Antibodies have been described that bind and neutralize both RSV and hMPV fusion (F) proteins.
RSV F protein is a type I integral membrane protein that is synthesized as a 574 amino acid inactive precursor, assembled into a trimer, post-translationally modified, then cleaved to produce F1, F2, and intervening peptide pep273. Functional F protein has both pre- and post-fusion conformations. RSV F protein is highly conserved among RSV isolates from both A and B subgroups3 and is the primary target for antiviral drug development3 with several antigenic regions capable of introducing neutralizing antibodies2. RSV and hMPV F protein share ~36% sequence similarity. Human monoclonal antibody (mAb) RSV-17E10 was generated against post-fusion RSV F using human hybridoma technology2. RSV-17E10 binds to pre- and post-fusion F proteins of RSV A strain A2 and RSV B strain 18537 and is capable of neutralizing RSV A2, RSV Long viruses (subgroup A), RSV 18537 B, and RSV WV/401R viruses (subgroup B). Competition-binding assay shows that RSV-17E10 targets antigenic site IV. The mode of action is independent of R429. RSV-17E10 is cross-reactive with hMPV F at antigenic site IV and neutralizes hMPV better than RSV. Cross-neutralization of RSV and hMPV is associated with recognition of the G430 and I432 residues, which are shared between RSV and hMPV F proteins. RSV-17E10 binds at a unique ~56o tilt, which may be a determinant of cross-reactivity with hMPV F. Antigen Distribution F protein is a surface glycoprotein. Research Area Infectious Disease . Respiratory Syncytial . Seasonal and Respiratory Infections . Viral . IVD Raw Material References & Citations1. National Center for Immunization and Respiratory Diseases (NCIRD), Division of Viral Diseases, Link Text
2. Mousa JJ, Binshtein E, Human S, et al. PLoS Pathog. 14(2):e1006837. 2018. 3. McLellan JS, Ray WC, Peeples ME. Curr Top Microbiol Immunol. 372:83-104. 2013. Technical ProtocolsCertificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.