Anti-Rhesus IgG2 (Clone 3C10.1) – Purified No Carrier Protein

IgG hinge region

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

3C10.1 activity is directed against rhesus macaque IgG2.

As part of preclinical vaccine development, particularly for HIV, Indian rhesus macaques (Macaca mulatta) are used to evaluate antibody-based correlates of vaccine-mediated protection¹. Four IgG subclasses are present in rhesus, but subclass numbering between rhesus and humans is not strictly consistent between species. Based on sequence alignment of the CH2 region responsible for FcγR recognition, rhesus subclasses are most similar to human IgG1. Most substitutions between rhesus and humans are located distal to the FcγR contact residues, except for H38 which borders an FcγR contact residue and is substituted to Q in all rhesus subclasses. Additionally, rhesus IgG2 has a deletion of P2, a residue proximal to the FcγR contact residues. Rhesus IgG2 binds to FcγR-conjugated fluorescently coded microspheres in a glycosylation dependent manner. Rhesus IgG2 and IgG3 can be specifically detected using antibodies raised against their hinges. In serum, rhesus IgG2 is the second most abundant Ig subclass, after IgG1. 3C10.1 was generated by immunizing mice with overlapping peptides derived from the IgG hinge region, VVHEPSNTKVDKTVGLPCRSTCPPCP and DKTVGLPCRSTCPPCPAELLGCPSVF¹. Administration of antibody to rhesus macaques results in a moderate reduction of CD8⁺ T cells in blood.

IgG2 is found in serum.

Boesch AW, Osei-Owusu NY, Crowley AR, et al. Front Immunol. 7:589. 2016.