Anti-RNA Polymerase II Antibody
Anti-RNA Polymerase II Antibody
Product No.: 70101
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Clone 8WG16 Target RNA Polymerase II Formats AvailableView All Product Type Monoclonal Alternate Names RNA polymerase II subunit B1, EC 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, EC 2.7.7.48 Isotype Mouse IgG2a Applications WB , AP |
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Antibody DetailsProduct DetailsReactive Species Eukaryotic Host Species Mouse Immunogen Purified wheat germ RNA polymerase II. Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco This antibody may be used to detect unproteolyzed RNA polymerase II or to inhibit specific transcription from class II promoters. This is a polyol-responsive antibody and can be used in gentle purifications of RNA polymerase II from from wheat germ, calf thymus, and yeast and is likely to purify RNA polymerase II from most eukaryotic organisms. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Monoclonal Antibody specific to RNA Polymerase II. It reacts with the highly conserved heptapeptide repeat of the
largest subunit of eukaryotic RNA polymerase II. It inhibits promoter directed transcription, but it does not inhibit elongation in the nonspecific
transcription assay. Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the sUniProtKB:P08775, PubMed:20231364, PubMed:23748380, PubMed:24207025, PubMed:26124092, PubMed:9852112}.; (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. {PubMed:18032511}. NCBI Gene Bank ID UniProt.org Research Area Protein Transcription References & CitationsThompson NE et al. 1989. J Biol Chem 264: 11511-11520 Thompson NE et al. 1990. J Biol Chem 265: 7069-7077. Technical Protocols AP Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
