Anti-RNase L – Purified
Anti-RNase L – Purified
Product No.: R6010
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Clone 2E9 Target RNaseL Formats AvailableView All Product Type Monoclonal Antibody Alternate Names 2',5'-oligoisoadenylate synthetase-dependent; 2-5A-dependent ribonuclease; 2-5A-dependent RNase; interferon-induced 2-5A-dependent RNase; Ribonuclease 4; Ribonuclease L; ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent); ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent); RNase L; PRCA1; RNS4 Isotype IgG1 κ Applications ELISA , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Human/Hamster/Primate Host Species Mouse Immunogen Purified, full-length, recombinant, human RNaseL produced in insect cells. Product Concentration 0.5 mg/ml Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This purified antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2831734 Applications and Recommended Usage? Quality Tested by Leinco WB ELISA Additional Applications Reported In Literature ? IHC FFPE Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 2E9 recognizes an epitope on human/hamster/primate RNaseL.
Background RNase L antibody, clone 2E9, recognizes the interferon (IFN)-inducible 2-5A-dependent ribonuclease L (RNase L), an endoribonuclease involved in antiviral signaling. It is widely expressed as a latent monomer in most mammalian tissues1. 2’,5’-oligoadenylate synthetase (OAS) is expressed following viral infection and exposure to IFN2. OAS proteins are activated by dsRNA, resulting in the production of 2’,5’-linked oligoadenylates (2-5A), which bind to latent RNase L, inducing dimerization and activation2. RNase L inhibits viral replication by cleaving viral and ribosomal RNA3,4 and inducing apoptosis in virus-infected cells5,6. RNase L is suggested to have antitumor effects, possibly through its pro-apoptotic activity7, and mutations in the RNASEL gene are associated with prostate cancer risk and progression8,9. Antigen Distribution RNase L is highly expressed in the spleen and thymus, followed by prostate, testis, uterus, small intestine, colon, and peripheral blood leukocytes. PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Immunology . Neuroinflammation . Neuroscience References & Citations1. Zhou A, et al. (2005) J Interferon Cytokine Res. 25(10):595-603 2. Silverman RH. (2007) Cytokine Growth Factor Rev. 18(5-6):381-8 3. Wreschner DH, et al. (1981) Nucleic Acids Res. 9(7):1571-81 4. Cooper DA, et al. (2014) Nucleic Acids Res. 42(8):5202-16 5. Castelli JC, et al. (1997) J Exp Med. 186(6):967-72 6. Zhou A, et al (1997) EMBO J. 16(21):6355-63 7. Xiang Y, et al. (2003) Cancer Res. 63(20):6795-801 8. Meyer MS, et al. (2010) Carcinogenesis. 31(9):1597-603 9. Silverman RH. (2003) Biochemistry. 42(7):1805-12 Technical Protocols IHC FFPE  Certificate of Analysis |
Formats Available
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Prod No. | Description |
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R6000 | |
R6010 | |
R6005 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
