Anti-SARS-CoV-2 Nucleocapsid (N) (Clone NP1-D4) – Biotin

SARS-CoV-2 Nucleocapsid (N) Protein

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Ships Overnight on Blue Ice

Anti-SARS-CoV-2 Nucleocapsid, clone NP1-D4, specifically targets an epitope on the SARS-CoV-2 nucleocapsid protein. Furthermore, it is reported to bind to the RNA binding domain of the N protein.

Coronavirus disease 2019 (COVID-19) is caused by the Coronaviridae family virus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)¹. SARS-CoV-2 has four structural proteins encoded by its single-stranded, positive-sense RNA genome: the spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins². The N protein is a highly conserved 46 kDa protein that shares 90% homology with SARS-CoV³. The N protein has an N-terminal (NTD) and C-terminal domain (CTD), which bind to RNA and self-oligomerize, respectively^4,5, forming a helical nucleocapsid structure within the viral envelope⁶. Other functions of the N protein included viral transcription, replication, and modulating cell signaling pathways^7,8. The N protein is abundantly expressed during infection, and antibodies^3,9 and memory T cells^10,11 targeting the N protein have been identified in convalescent sera. Therefore, the N protein is a target in some vaccines and diagnostic assays¹². The N protein also has therapeutic potential, as it evades RNAi-mediated antiviral responses¹³.

The nucleocapsid protein is expressed in the internal nucleocapsid of SARS-CoV-2.

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2. Wu, F., Zhao, S., Yu, B. et al. Nature 579, 265–269. 2020.
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4. Kang S, Yang M, Hong Z, et al. Acta Pharm Sin B. 10.1016/j.apsb.2020.04.009. 2020.
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9. To K.K., Tsang O.T., Leung W.S., et al. Lancet Infect. Dis. 2020.
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11. Ni L, Ye F, Cheng ML, et al. Immunity. 52(6):971-977.e3. 2020.
12. Liu L, Liu W, Zheng Y, et al. Microbes Infect. 22(4-5):206-211. 2020.
13. Mu J, Xu J, Zhang L, et al. Sci China Life Sci. 1-4. 2020.