Anti-SARS-CoV Spike (17aa – Intermediate Domain)

Anti-SARS-CoV Spike antibody was raised against a peptide corresponding to 17 amino acids near the center of SARS-CoV Spike glycoprotein. The immunogen is located within amino acids 550-600 of SARS-CoV Spike.

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti- Severe Acute Respiratory Syndrome Spike (SARS-CoV Spike) recognizes an epitope in the intermediate domain of SARS Spike. This polyclonal antibody was purified using affinity chromatography.

A novel coronavirus has recently been identified as the causative agent of SARS-CoV.^1-2 Coronaviruses are a major cause of upper respiratory diseases in humans.³ The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.⁴

1. Marra, M. A. et al. (2003) Science 300:1399 2. Rota, P. A. et al. (2003) Science 300:1394 3. Navas-Nartin, S. R. et al. (2004) J. Neurovirol. 10:75 4. Li, W. et al. (2003) Nature 426:450