Anti-Sodium Iodide Symporter Antibody (11563)
Anti-Sodium Iodide Symporter Antibody (11563)
Product No.: 11563
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Clone FP5 Target Sodium Iodide Symporter Formats AvailableView All Product Type Monoclonal Alternate Names Na(+/I(- cotransporter, Sodium-iodide symporter, Na(+/I(- symporter, Solute carrier family 5 member 5 Isotype Mouse IgG1 Applications IHC , WB , AM , ICC/IF |
Data
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Immunohistochemistry analysis using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Western Blot analysis of Human thyroid lysate showing detection of Sodium Iodide Symporter protein using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (11563). Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody (11563) at 1:1000. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Fusion protein consisting of aa468-643 of human NIS attached to mannose binding protein (MBP). Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL. A major band of ~97kDa is detected. Depending on the sample, some minor bands may be detected as well. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human, mouse
and rat NIS. Background The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells. This is an important step in the process of iodide organification and the formation of triiodothyronine and thyroxine. The importance of NIS is related to its role in thyroid function, the pathogenesis of thyroid disease, and its expression (or lack of expression) in thyroid malignancies. It has also been suggested that NIS may represent an autoantigen in autoimmune disease of the thyroid. Antigen DetailsFunction Mediates iodide uptake in the thyroid gland. NCBI Gene Bank ID UniProt.org Research Area Ion Channels References & CitationsTechnical Protocols  AM ICC/IF |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
