Anti-Superoxide dismutase 1 Antibody (13065)
Data
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (13065). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (13065) at 1:110 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD1 (UBB) Antibody. (C) Composite.
Western blot analysis of Human, Rat, Mouse Hela cells, Brain, Lung showing detection of ~18 kDa SOD1 (UBB) protein using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (13065). Lane 1: Molecular Weight Ladder. Lane 2: Human HeLa Cell Lysates. Lane 3: Rat Brain. Lane 4: Mouse Lung. Load: 20 µg. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody (13065) at 1:1000. Predicted/Observed Size: ~18 kDa. - -
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide corresponding to unfolded beta barrel (UβB) region at the N-terminus of SOD1 Product Concentration Lot Specific Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by protein A affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Regulatory Status For in vitro investigational use only. Not intended for diagnostic or therapeutic applications. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1ug/mL. A band of ~18kDa is detected.
Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Superoxide dismutase 1 (SOD1) Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems. {PubMed:24140062}. NCBI Gene Bank ID UniProt.org Research Area Enzymes References & CitationsTechnical Protocols  ICC/IF Certificate of Analysis |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
