Anti-T2A tag (Clone 3H4-3A5) – Purified No Carrier Protein
Anti-T2A tag (Clone 3H4-3A5) – Purified No Carrier Protein
Product No.: T753
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Clone 3H4-3A5 Target T2A tag Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names 2A peptide tag Isotype Mouse IgG1 κ Applications IF , IP , WB |
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Antibody DetailsProduct DetailsReactive Species Epitope Tag Host Species Mouse Immunogen KLH-coupled synthetic peptide corresponding to 2A peptide of Thosea asigna virus Product Concentration ≥1.0 mg/ml Purity ≥90% monomer by analytical SEC and SDS-Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. State of Matter Liquid Storage and Handling This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? IF, IP, WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 3H4-3A5 activity is directed against the T2A tag (CGDVEENPG). Background 2A viral peptide allows robust production of several proteins in a near-stochiometric manner
from the same RNA1 by acting as a cis-acting hydrolase element (CHYSEL)2. When ribosomes encounter the 2A viral peptide conserved consensus sequence (Asp-Val/Ile-Glu-X-Asn-Pro-Gly-Pro), a “ribosomal-skip” or “STOP&GO” occurs and a polypeptide is released while translation continues1,2. In this way, multiple proteins can be produced from a single recombinant construct. This system can be used for multicistronic expression in a wide variety of eukaryotic species2. Its efficacy has been demonstrated in many cell types and can be inherited through the mouse germline. 2A peptide was identified in the Aphthoviruses subgroup of the picornavirus family2. In these viruses, a large polyprotein produces multiple proteins from a single open reading frame, with 2A peptide mediating co-translational cleavage between the Gly and Pro at the end of the peptide. The 2A peptide remains fused to the C-terminus of the upstream protein after cleavage. The Pro is added to the N-terminus of the downstream protein. Translation termination release factors eRF1 and eRF3 play a role in release. Of the different 2A peptides available, T2A has the highest cleavage efficiency3. T2A is derived from Thosea asigna virus. 3H4-3A5 is a recombinant peptide antibody developed against a KLH-coupled synthetic peptide corresponding to Thosea asigna virus 2A peptide (CGDVEENPG). Antigen Distribution T2A is used to produce polycistronic recombinant proteins in a wide variety of eukaryotes.
Research Area Cell Biology References & Citations1 Ahier A, Jarriault S. Genetics. 196(3):605-613. 2014. 2 Trichas G, Begbie J, Srinivas S. BMC Biol. 6:40. 2008. 3 Chng J, Wang T, Nian R, et al. MAbs. 7(2):403-412. 2015. Technical ProtocolsIF   Certificate of Analysis |
Formats Available
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
