Anti-Transmissible Gastroenteritis Mouse Monoclonal Antibody – Library Pack (180101)

Transmissible Gastroenteritis (TGEV) is an enveloped Coronavirus with a positive-sense single- stranded RNA genome and a helical symmetry. The genomic size of coronaviruses is approximately 28.6 kilobases. Proteins that contribute to the overall structure of TGEV include the spike (S), envelope (E), membrane (M) and nucleocapsid (N). Other coronaviruses belonging to this family include Feline coronavirus (FeCV), Canine coronavirus (CCV), and Feline infectious peritonitis virus (FIPV). TGEV infects pigs. However, in young pigs, the mortality rate is close to 100%. The pathology of TGEV is similar to that of other coronaviruses. Coronaviruses enter the host by first attaching to the host cell using the spike glycoprotein. The S protein interacts with the porcine aminopeptidase N (pAPN), a cellular receptor, to aide in its entry. A domain in the S spike protein is recognized by pAPN, and transfection of pAPN occurs to nonpermissive cells and infects them with TGEV. Once the virus infects the host, it multiplies in the cell lining of the small intestine resulting in the loss of absorptive cells that in turn leads to shortening of villi. The infected swine then has reduced capability for digesting food and die from dehydration.

TGEV-infected ST cells

These antibodies recognize TGEV and do not cross-react with FIPV, FeCV, or CCV. #18003 and #18004 can neutralize TGEV in vitro.

Anti-Transmissible Gastroenteritis Mouse Monoclonal Antibody Library Pack - Includes 100 ug of each of the following antibodies:

18001 - Anti-Transmissible Gastroenteritis Mouse Monoclonal Antibody - Clone TGEV10-116.3.3 - Isotype IgG1

18002 - Anti-Transmissible Gastroenteritis Mouse Monoclonal Antibody - Clone TGEV11-314.6 - Isotype IgG1

18003 - Anti-Transmissible Gastroenteritis Mouse Monoclonal Antibody - Clone TGEV15-886.6 - Isotype IgG2a

18004 - Anti-Transmissible Gastroenteritis Mouse Monoclonal Antibody - Clone TGEV15-948.2.1 - Isotype IgG2a

Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles.