Anti-Tumor Necrosis Factorα Antibody (21301)

Anti-Tumor Necrosis Factorα Antibody (21301)

Product No.: 21301


Clone 2C8 Target Tumor Necrosis Factor-α Formats AvailableView All Product Type Monoclonal Alternate Names Cachectin, TNF-α, Tumor necrosis factor ligand superfamily member 2, TNF-a [Cleaved into: Tumor necrosis factor, membrane form Isotype Mouse IgG1 Applications ELISA , IHC


Select Product Size 200 µg — $361.00	Qty Max: Min: 1 Step: 1 $361.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Recombinant human TNFa. Product Concentration Lot Specific Formulation PBS containing 0.1% sodium azide State of Matter Liquid Product Preparation Purified by Protein A affinity chromatography Storage and Handling These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco These antibodies may be used in immunoassays or in immunohisto- chemistry to detect and/or quantitate TNFa. 21301 and 21302 may be used in biological assays to inhibit activity of TNFa. Other applications are under investigation. Additional Applications Reported In Literature ? ELISA, IHC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity 21303 recognizes a different epitope on TNFα than 21301 and 21302. 21301 and 21302 can inhibit the biological activity of recombinant and native TNFα. Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819). {UniProtKB:P06804, PubMed:12794819, PubMed:16829952, PubMed:22517918, PubMed:23396208}.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. {PubMed:16829952}. NCBI Gene Bank ID TNF UniProt.org P01375 Research Area Growth Factors, Cytokines, Receptors References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Recombinant human TNFa.

Product Concentration

Lot Specific

Formulation

PBS containing 0.1% sodium azide

State of Matter

Liquid

Product Preparation

Purified by Protein A affinity chromatography

Storage and Handling

These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

These antibodies may be used in immunoassays or in immunohisto- chemistry to detect and/or quantitate TNFa. 21301 and 21302 may be used in biological assays to inhibit activity of TNFa. Other applications are under investigation.

Additional Applications Reported In Literature ?

ELISA,
IHC

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

21303 recognizes a different epitope on TNFα than 21301 and 21302. 21301 and 21302 can inhibit the biological activity of recombinant and native TNFα.

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819). {UniProtKB:P06804, PubMed:12794819, PubMed:16829952, PubMed:22517918, PubMed:23396208}.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. {PubMed:16829952}.

NCBI Gene Bank ID

TNF

UniProt.org

P01375

Research Area

Growth Factors, Cytokines, Receptors