Anti-VaChT Transporter Antibody (56513)
Anti-VaChT Transporter Antibody (56513)
Product No.: 56513
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Clone S6-38 Target VaChT Transporter Formats AvailableView All Product Type Monoclonal Alternate Names VAChT, Solute carrier family 18 member 3 Isotype Mouse IgG1 Applications IHC , WB , ICC/IF |
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38 (56513). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody (56513) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VAChT Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38 (56513). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody (56513) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VAChT Antibody. (D) Composite.
Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38 (56513). Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody (56513) at 1:1000. - -
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Synthetic peptide corresponding to aa 521-532 (CEDDYNYYYTRS) of human VAChT (accession no. NP_003046) Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for
use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-10ug/mL. A band of 55kDa is detected. The molecular weight of glycosylated VAChT is 70kDa.
Immunohistochemistry: use at 1-10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human, mouse,
and rat VAChT. Background VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene. Function Involved in acetylcholine transport into synaptic vesicles. {PubMed:8071310}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & CitationsTechnical Protocols  ICC/IF Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
