Chlamydia pneumoniae COMC Antigen (Chlamydia Outer Membrane Complexes) Antigen (BA1371VS)

The genus Chlamydia includes, among others, the human and animal pathogenic species C. pneumoniae, C. trachomatis and C. abortus. For the production of the native antigens, strains C. pneumoniae TWAR strain TW-183, C. trachomatis Lymphogranuloma venereum (LGV II) strain 434 and C. abortus strain B-577 are cultivated in cell culture (Vero or BGM cells) and the elementary and reticular bodies are prepared by ultra-centrifugation. In case of C. pneumoniae a further purified native antigen is available – the COMC premium antigen.

Liquid

0.1 M glycine buffer pH 9.5, containing 10 mM dithiothreitol.

This antigen is prepared by detergent extraction of the reticular bodies to prepare chlamydial outer membrane complexes (COMCs). This premium antigen is highly recommended for the detection of IgG, IgM and IgA antibodies against C. pneumoniae. Additionally a recombinant C. trachomatis MOMP antigen is also available which is produced in E. coli, purified by chromatography in the presence of urea. Purity of ≥ 95 % is confirmed via SDS-PAGE (Coomassie staining) and the antigen is suitable for the detection of IgG, IgM and IgA antibodies against C. trachomatis.

Store at -65°C or lower. Avoid repeated freeze-thaw cycles. Sonicate before use. 10 years from date of manufacture (under recommended storage conditions).

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