Goat Anti-Human IgG (H&L) (Adsorbed Against: Ms., Bv., Hs.) – Biotin

Purified Human IgG (H&L) (>98%)

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Biotin conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.

One year from date of reconstitution

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It is recommended that working dilutions be prepared fresh daily.

WB: 1:20,000 - 1:400,000
ELISA: 1:20,000 - 1:400,000
IHC: 1:500 - 1:5,000
FC: 1:200 - 1:1,000
IF: 1:200 - 1:1,000

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

Goat Whole Molecule Anti-Human IgG (H&L) recognizes Human IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.