Human IgG4 Isotype Control – Purified No Carrier Protein

Human IgG4 Isotype Control – Purified No Carrier Protein

Product No.: LT9020

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Product No.LT9020
Clone
MOPC21.4
Product Type
Recombinant Isotype Control
Isotype
Human IgG4κ
Applications
FA
,
FC

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Select Product Size
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Antibody Details

Product Details

Expression Host
HEK-293 Cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 0.5 EU / ml as determines by the LAL method
Purity
≥95% by SDS Page
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
This isotype control antibody should be used at the same concentration as the primary antibody.
Applications and Recommended Usage?
Quality Tested by Leinco
FC Refer to specific primary antibody of interest for concentration usage
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This Human IgG4 isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this Low Endotoxin Functional Formulation is suitable for in vivo applications.

References & Citations

FA
Flow Cytometry

Certificate of Analysis

Formats Available

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.