Mouse IgG2a Fc Muted™ (D265A), Isotype Control – Purified No Carrier Protein
Mouse IgG2a Fc Muted™ (D265A), Isotype Control – Purified No Carrier Protein
Product No.: C581
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Product No.C581 Clone C1.18.4 (D265A) Target KLH Product Type Recombinant Isotype Control Alternate Names C1.18.4 (D265A) Isotype Mouse IgG2a k Applications FA , FC , in vivo |
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Antibody DetailsProduct DetailsExpression Host HEK-293 Cells FC Effector Activity Muted Immunogen KLH Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥95% by SDS Page ⋅ ≥95% monomer by analytical SEC Formulation This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. State of Matter Liquid Product Preparation Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -80°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? FC, FA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Mouse IgG2a (Anti-KLH) isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissue. Research Area Cell Biology References & Citations1. Ikeda Y. et al. (2003) Blood.101(2):621-3. Article Link 2. Wright, TM et al. (2000) J Immunol.164(12):6138-46.PubMed 3. Wright, TM et al. (2001) Arthritis Rheum. 44(7):1654-9. PubMed 4. Nishikawa, Takeji et al. (2000) Journal of Investigative Dermatology 114(1): 88-94. PubMed Technical ProtocolsCertificate of Analysis |
Formats Available
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Prod No. | Description |
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C581 |
