Mouse IgG2a Fc Muted™ (D265A), Isotype Control – Purified No Carrier Protein

Mouse IgG2a Fc Muted™ (D265A), Isotype Control – Purified No Carrier Protein

Product No.: C581

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Product No.C581
Clone
C1.18.4 (D265A)
Target
KLH
Product Type
Recombinant Isotype Control
Alternate Names
C1.18.4 (D265A)
Isotype
Mouse IgG2a k
Applications
FA
,
FC
,
in vivo

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Select Product Size
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Antibody Details

Product Details

Expression Host
HEK-293 Cells
FC Effector Activity
Muted
Immunogen
KLH
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥95% by SDS Page
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
FC,
FA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This Mouse IgG2a (Anti-KLH) isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissue.
Research Area
Cell Biology

References & Citations

1. Ikeda Y. et al. (2003) Blood.101(2):621-3. Article Link
2. Wright, TM et al. (2000) J Immunol.164(12):6138-46.PubMed
3. Wright, TM et al. (2001) Arthritis Rheum. 44(7):1654-9. PubMed
4. Nishikawa, Takeji et al. (2000) Journal of Investigative Dermatology 114(1): 88-94. PubMed
FA
Flow Cytometry
in vivo Protocol

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.