Anti-Mouse LPAM-1 – Purified
Anti-Mouse LPAM-1 – Purified
Product No.: L291
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Clone DATK32 Target Integrin α4β7 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names α4β7 Integrin, CD49d/β7, LPAM-1, ITGA4, ITGB7 Isotype IgG2a κ Applications B , FC , IHC FF , IP |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen TK1 Cells Product Concentration 0.5 mg/ml Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Product Preparation The antibody was purified by affinity chromatography. Storage and Handling This purified antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2831200 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this DATK32 antibody for staining cells in flow cytometry is ≤1.0 µg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? B This antibody has been reported to block α4β7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin for In vitro and In vivo studies.
IHC FF IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone DATK32 recognizes an epitope specific to the mouse LPAM-1 heterodimer. Background LPAM-1 antibody, clone DATK32, recognizes mouse lymphocyte Peyer’s patch adhesion molecule (LPAM-1), also known as integrin alpha 4 beta 7 (α4β7). LPAM-1 is a heterodimer of the 154 kDa integrin α4 (CD49d) and 130 kDa integrin β7 chains and is a member of the Ig superfamily. LPAM-1 is expressed on most peripheral lymphocytes, subsets of thymocytes, and bone marrow hematopoietic stem cells1,2. LPAM-1 mediates adhesion to endothelial cells, promoting leukocyte transmigration across high endothelial venules (HEVs) during the inflammatory response. LPAM-1 binds to several ligands, including vascular adhesion molecule-1 (VCAM-1/CD106), mucosal addressin cell adhesion molecule-1 (MAdCAM-1), and fibronectin. MAdCAM-1 is expressed exclusively on endothelial cells of the gut and gut-associated lymphoid tissues, such as Peyer’s patches (PPs), and binding of LPAM-1 to MAdCAM-1 contributes to lymphocyte homing to mucosal tissues3,4. Vedolizumab, an anti-LPAM-1 monoclonal antibody, is approved to treat inflammatory bowel disease and effectively induces and maintains remission in Crohn's disease and ulcerative colitis5-8.
Antigen Distribution LPAM-1 is expressed on most peripheral lymphocytes, subsets of thymocytes, and bone marrow progenitor cells.
Ligand/Receptor VCAM-1 (CD106), MAdCAM-1, fibronectin Function Lymphocyte adhesion PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Immunology . Neuroscience . Synaptic Biology References & Citations1. Andrew DP, et al. (1996) Eur J Immunol. 26(4):897-905 2. Murakami JL, et al. (2016) Stem Cells Dev. 25(1):18-26 3. Hu MC, et al. (1992) Proc Natl Acad Sci USA. 89(17):8254-8 4. Hamann A, et al. (1994) J Immunol. 152(7):3282-93 5. Sandborn WJ, et al. (2013) N Engl J Med. 369:711–721 6. Feagan BG, et al. (2013) N Engl J Med. 369:699–710 7. Kopylov U, et al. (2019) Dig Liver Dis. 51:68–74 8. Danese S, et al. (2019) Gastroenterology.157:1007–1018 e1007 Technical ProtocolsB  IHC FF  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
