Normal Mouse Serum (Filtered)

Product No.: S5001

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Product Type
Normal Serum
Applications
B
,
ICC
,
IHC
,
IHC FF
,
IHC FFPE

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Select Product Size
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Product Details

Host Species
Mouse
Storage and Handling
Store at -20°C or -80°C.
Formulation
Whole normal serum from unimmunized animals of mixed sex. Does not contain any preservatives or anticoagulants.
Country of Origin
USA
Shipping
Shipped on Dry Ice. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

Description

Background
Normal mouse serum is used in research for preventing unwanted antibody interactions during tissue and cell staining experiments.

It blocks two main issues:
1) Fc receptor binding- The serum blocks Fc receptors on the sample from attaching to the primary and secondary antibodies used in the experiment.
2) Non-specific antibody binding- It also prevents the antibodies themselves from sticking to irrelevant parts of the sample.

What to look for when choosing a blocking serum:
1) Species matters- The serum typically comes from a different animal species than the one used to create the primary antibody.
2) Secondary antibody consideration- Often, the blocking serum is derived from the same species as the secondary antibody.

This serum can be used in two ways:
1) Direct application- The serum is applied directly to the sample for blocking.
2) Blocking buffer component- The serum can be incorporated into a solution specifically designed for blocking, called a blocking buffer.

Directions for Use
Normal serum diluted to 5% (v/v) in PBS (or comparable buffer) is strongly recommended as a blocking agent to reduce background from non-specific, conserved-sequence, and/or Fc-receptor binding.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Related Protocols

B
ICC
IHC
IHC FF
IHC FFPE
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.