Proliferating Cell Nuclear Antigen (12502P)

Sterile-filtered solution in 20mM Tris, pH 7.5, 2mM EDTA, and 20% glycerol.

Liquid

Although stable at 4°C for 4 weeks, product is best stored at or below -20°C. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage. For in vitro investigational use only.

Next Day 2-8°C

Recombinant Human Proliferating Cell Nuclear Antigen

PCNA is located in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps in processing of leading strand synthesis during DNA replication. In reaction to DNA damage, PCNA is ubiquitinated and participates in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA gene. PCNA is expressed during late G1 phase, S phase of mitosis, and persists until the end of the M phase. PCNA can be induced by UV irradiation.

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs rPubMed:18719106, PubMed:19443450, PubMed:24695737, PubMed:24939902}.