Recombinant Human Activin RIIA

Activin A receptor, type IIA, also known as ACVR2A, is a human gene.¹ ACVR2A is an activin type 2 receptor. This gene encodes activin A type II receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

The biological activity of Human Act R-IIA was determined by its ability to inhibit Activin A induced hemoglobin expression in K562 human chronic myelogenous leukemia cells. ¹ Approximately 0.03 - 0.1 μg/ml of rhActivin RIIA will inhibit 50% of the biological response due to 3 ng/ml of rhActivin A.

Fc Fusion Protein

setqec lffnanwekd rtnqtgvepc ygdkdkrrhc fatwknisgs ieivkqgcwl ddincydrtd cvekkdspev yfcccegnmc nekfsyfpem evtqptsnpv tpkpiegrmd pkscdkthtc ppcpapellg gpsvflfppk pkdtlmisrt pevtcvvvdv shedpevkfn wyvdgvevhn aktkpreeqy nstyrvvsvl tvlhqdwlng keykckvsnk alpapiekti skakgqprep qvytlppsrd eltknqvslt clvkgfypsd iavewesngq pennykttpp vldsdgsffl yskltvdksr wqqgnvfscs vmhealhnhy tqkslslspg khhhhhh

Ser25

Lyophilized

The predicted molecular weight of Recombinant Human Act R-IIA is Mr 40 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 50 kDa.

40

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Schwall, RH. et al. (1991) Method Enzymol. 198:340.