Recombinant Human CD80

CD80 is a highly glycosylated 60 kD protein that is part of the Ig superfamily and is significantly involved in immune cell activation in response to pathogens. CD80 is closely related to, and works in tandem with CD86 (B7-2) to prime T- cells. CD80 binds to CTLA-4 to deliver an inhibitory signal to T cells. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. It is thought that CD80 interacts with a ligand on Natural Killer cells, activating the Natural Killer cell-mediated cell death of the CD80 carrier. The activation of Natural Killer cell-mediated death via CD80 interactions has potential as a possible cancer immunotherapy through the induction of CD80 expression on tumor cells.

Measured by its binding ability in a functional ELISA, when Recombinant Human B7-1/CD80 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CTLA-4 that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.

His-tag

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Val35

Lyophilized

The predicted molecular weight of Recombinant Human B7-1 is Mr 25 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 45-58 kDa.

25

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Abbas, AK. et al. (1999) Nature Rev. Med. 5:1345
2. Sharpe, AH. et al. (2002) Nature Rev. Immunol. 2:116
3. Chang, TT. et al. (2002) Curr. Dir. Autoimmun. 5:113