Recombinant Human B7-H2

B7-H2, also known as ICOSL and B7RP-1, is a transmembrane glycoprotein and member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. B7-H2 is expressed on B cells and macrophages and binds to ICOS on activated T cells delivering a positive co-stimulatory signal for optimal T cell function (1). The structural features of B7-H2 are crucial for its co-stimulatory function where receptor binding is mediated solely by the IgV domain but requires the IgC domain for maintaining the structural integrity of the protein (2).

Fc Fusion Protein

dt qekevramvg sdvelscacp egsrfdlndv yvywqtsesk tvvtyhipqn sslenvdsry rnralmspag mlrgdfslrl fnvtpqdeqk fhclvlsqsl gfqevlsvev tlhvaanfsv pvvsaphsps qdeltftcts ingyprpnvy winktdnsll dqalqndtvf lnmrglydvv svlriartps vnigccienv llqqnltvgs qtgndigerd kitenpvstg eknaatwsdi egrmdpkscd kthtcppcpa pellggpsvf lfppkpkdtl misrtpevtc vvvdvshedp evkfnwyvdg vevhnaktkp reeqynstyr vvsvltvlhq dwlngkeykc kvsnkalpap iektiskakg qprepqvytl ppsrdeltkn qvsltclvkg fypsdiavew esngqpenny kttppvldsd gsfflysklt vdksrwqqgn vfscsvmhea lhnhytqksl slspgk

Asp19

Lyophilized

The predicted molecular weight of Recombinant Human B7-H1 is Mr 53.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 80-90 kDa.

53.4

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Wiendl, H. et al. (2003) Brain 126:1026
2. Yoshinaga, SK. et al. (1999) Nature 402:827
3. Chattopadhyay, KJ. et al. (2006) Immunol. 177:3920