Recombinant Human BAFF-R

BAFF receptor (BAFF R/TNFRSF13/BlyS) is a type III transmembrane protein and member of the TNF receptor (TNFR) super family. It is highly expressed in spleen, lymph node, resting B cells, peripheral blood leukocytes and to some extent, in activated B cells and resting CD4+ cells. BAFF R plays a role in nearly all stages of differentiation of B cells from development to the maintenance of long-lived bone marrow plasma cells. ¹ BAFF R is one of three receptors, including TACI and BCMA, identified for BAFF. BAFF R signals by activation of the NFκB signaling pathway.² Overexpression of the BAFF R ligand, BAFF, in mice leads to autoimmune characteristics similar to systemic lupus erythematosus (SLE) and Sjogren’s syndrome. Furthermore, high levels of BAFF have been detected in the serum of humans with SLE, rheumatoid arthritis, Sjogren’s syndrome and systemic sclerosis.

The biological activity of Human BAFF Receptor is determined by its ability to block mouse BAFF induced mouse splenocyte survival.^3,4,5 The expected ED₅₀ for this effect is 4.0 µg/ml in the presence of 1 µg/ml of human soluble BAFF.

Fc Fusion Protein

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Ser7

Lyophilized

The predicted molecular weight of Recombinant Human BAFF-R is Mr 33 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 40-50 kDa.

34

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

1. Thompson, JS. et al. (2001) Science 293:2108
2. Mackay, F. et al. (2002) Nature Rev. Immunol. 2:464
3. Moore, PA. et al. (1999) Science 285:260
4. Gross, JA. et al. (2000) Nature 404:995
5. Schneider, P. et al. (1999) J. Exp. Med. 189:1747