Recombinant Human BDNF

Brain-derived neurotropic factor (BDNF), also known as MGC34632, is a member of the NGF family of neurotrophic growth factors that includes NGF, NT-3, and NT-4/5. Like other members of this family, BDNF supports neuron proliferation and survival (1). It acts on certain neurons of the central and peripheral nervous systems, helping to support the survival of existing neurons and encourage the growth and differentiation of new neurons and synapses. In the brain, BDNF is active in the hippocampus, cortex, and basal forebrain, areas vital to learning, memory and higher thinking (2). Despite its name, BDNF is actually found in a range of tissue and cell types, not just in the brain. It is also expressed in the retina, the CNS, motor neurons, the kidneys and the prostate. BDNF binds at least two receptors on the surface of cells which are capable of responding to this growth factor, TrkB and the LNGFR (3). Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients (4-5). In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart (6). Various studies have shown possible links between BDNF and other conditions, such as depression, schizophrenia, obsessive-compulsive disorder, Rett syndrome, dementia, anorexia nervosa, bulimia nervosa, epilepsy and eczema.

The activity is determined by the dose-dependent proliferation of C6 cells and is typically between 0.5-1.5 μg/mL (1 x 103 units/mg).

MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Lyophilized

Noncovalently linked homodimer

Non-glycosylated homodimer, containing two 119 amino acid chains with a molecular weight of Recombinant Human BDNF is Mr 27 kDa.

This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one (1) week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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