Recombinant Human Cardiotrophin-1

Human CT-1 is a member of the IL-6 family of cytokines which also includes LIF, CNTF, OSM (Oncostatin M), IL-11, IL-6 and possibly NT-1/ BSF-3. CT-1 is a pleiotropic cytokine which signals through the LIF receptor and the gp130 receptor subunit. CT-1 has the ability to induce cardiac myocyte hypertrophy, and enhances the survival of cardiomyocyte and different neuronal populations. Biologically active human CT-1 is synthesized as a 201 amino acid polypeptide lacking a hydrophobic N-terminal secretion signal sequence.

Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity.

The biological activity of Human CT-1 was determined in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323). The expected ED₅₀ is typically 1 - 4 ng/ml.

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Ser2

Solution

The predicted molecular weight of Recombinant Human CT-1 is Mr 21 kDa.

21

This recombinant protein was 0.2 µm filtered and is supplied in a solution of 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This protein is stable for twelve months when stored at -20°C to -70°C. After thawing and addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for long term storage aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Dry Ice