Recombinant Human CD30 Ligand

The CD30 ligand (CD30L), also known as CD153 and TNFSF8, is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (1). CD30L is expressed on the cell surface of activated T cells, B cells, stimulated monocyte-macrophages, granulocytes, eosinophils and some Burkitt-like lymphoma cell lines (2). Recombinant CD30L has been shown to co-stimulate T cells and to act as a mitogen for Hodgkin's disease derived cell lines (3). The receptor for CD30L is a type I cytokine receptor that is highly expressed by activated T cells, Hodgkin and Reed- Sternberg (H-RS) cells, and anaplastic large cell lymphoma cells (4). CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis (5).

The biological activity of Human CD30L was determined by its ability to stimulate IL-6 secretion by HDLM cells, a Hodgkin’s lymphoma cell line (Gruss, H-J. et al., 1996, Blood 87:2443). The expected ED₅₀ for this effect is typically 0.1 - 0.4 μg/mL in the presence of 10 μg/mL of a cross-linking antibody PN:R1251.

Fc Fusion Protein

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Met

Lyophilized

The predicted molecular weight of Recombinant Human CD30 Ligand is Mr 22 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 30-40 kDa.

22

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Croager, EJ. et al. (1997) Biochim. Biophys. Acta. 1353:231
2. Gruss, HJ. et al. (1994) Blood 83:1521
3. Wendtner, CM. et al. (1995) Cancer Res. 55:4157
4. Fischer, M. et al. (2006) J. Clin. Invest. 116:2748
5. Blazar, BR. et al. (2004) J. Immunol. 173:2933