Recombinant Human Connective Tissue Growth Factor
Recombinant Human Connective Tissue Growth Factor
Product No.: C727
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Alternate Names CCN2, Hypertrophic Chondrocyte-Specific Protein 24 [HCS24], IGFBP8, MGC102839, NOV2 Product Type Recombinant Protein Expression Host E. coli Cells Species Human Applications ELISA ⋅ IF Microscopy ⋅ IP |
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BackgroundCTGF (connective tissue growth factor) is a cysteine-rich, matrix-associated, heparin-binding protein and a member of the CCN family of secreted cysteine rich regulatory proteins and is the major mitogenic and chemoattractant protein produced by umbilical vein and vascular endothelial cells. CTGF stimulates the proliferation and differentiation of chondrocytes, induces angiogenesis, promotes cell adhesion of fibroblasts, endothelial, and epithelial cells, and binds to IGF, TGF beta1, and BMP-4. Cell migration and adhesion are signaled through binding to specific cell surface integrins and to heparin sulfate proteoglycans CTGF, a lower molecular weight isoform containing the C-terminal portion of the full length CTGF protein, exerts full heparin binding, cell adhesion, and mitogenic CTGF activity. Protein DetailsPurity >98% by SDS-PAGE and HPLC Endotoxin Level <1.0 EU/µg as determined by the LAL method Biological Activity The biological activity of Human Connective Tissue Growth Factor is determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED<sub>50</sub> for this effect is 1.0-2.0 µg/ml. Amino Acid Sequence MGKKCIRTPK ISKPIKFELS GCTSMKTYRA KFCGVCTDGR CCTPHRTTTL PVEFKCPDGE VMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA State of Matter Lyophilized Storage and Stability The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day Ambient References & Citations1. Fine, HA. et al. (2011) J Natl Cancer Inst. 103(15): 1162–1178.PubMed Technical ProtocolsCertificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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