Recombinant Human CXCL16 (Extracellular Domain)

CXC chemokine ligand 16 (CXCL16), also known as SR-PSOX, is a 20 kDa type I transmembrane protein. It acts as a scavenger receptor on macrophages, which specifically binds to oxidized low density lipoprotein, suggesting that it may be involved in pathophysiology such as atherogenesis. (1) CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. It is found in liver tissue and influences the uptake, subcellular localization and cytokine profile induced by D oligonucleotides (2-3). It is produced by dendritic cells found in the T cell zones of lymphoid organs and by cells found in the red pulp of the spleen (4). Cells that bind and migrate in response to CXCL16 include several subsets of T cells and natural killer T (NKT) cells (4). Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. It may play a pro-inflammatory role in Inflammatory Bowel Disease (IBD), particularly Crohn's disease (5).

The biological activity of Human CXCL16 (Extracellular Domain) was determined by its ability to inhibit rmβ-NGF-induced proliferation of TF-1 cells. The expected ED₅₀ is typically 0.5 - 1.5 μg/mL in the presence of 2 ng/mL of rmβ-NGF.

Histidine Tag

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Asn49

Lyophilized

The predicted molecular weight of Recombinant Human CXCL16 (Extracellular Domain) is Mr 20 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 40-45 kDa.

20

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Mummidi, S. et al. (2004) J. Biol. Chem. 279:3188
2. Adams, DH. et al. (2005) J. Immunol. 174:1055
3. Clinman, DM. et al. (2006) J. Immunol. 177:1575
4. Matloubian, M. et al. (2000) Nat. Immunol. 1:298
5. Broedl, UC. et al. (2008) Scand. J. Gastroenterol. 43:283