Recombinant Human CXCL5/ ENA-78 (5-78 a.a.)
Recombinant Human CXCL5/ ENA-78 (5-78 a.a.)
Product No.: E151
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Alternate Names Chemokine (C-X-C Motif) Ligand 5, AMCF-II, Epithelial-Derived Neutrophil-Activating Peptide 78 (ENA-78), GCP-2, Scyb5, Scyb6, Lipopolysaccharide-Induced CXC Chemokine (LIX) Product Type Recombinant Protein Expression Host E. coli Cells Species Human |
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BackgroundC-X-C motif chemokine 5 (CXCL5), also known as ENA-78 and SCYB5, is an inflammatory chemokine that belongs to the CXC chemokine family. It is a potent chemotaxin involved in neutrophil activation and is expressed in monocytes, platelets, endothelial cells and mast cells (1). CXCL5 elicits biological activities by signaling through the cell surface chemokine receptor CXCR2 (2). It has been shown to be cleaved by MMP-12 at the ELR sequence motif, which is known to be the critical receptor binding motif (3). CXCL5 is produced concomitantly with IL-8 in response to stimulation with either IL-1 or TNFα and can be induced by exposure to bacterial LPS (4, 5). Expression of CXCL5 has been observed in eosinophils and can be inhibited with the type II interferon IFN-γ. It is expressed by epithelial cells within colorectal mucosa and has been implicated in Crohn's disease and ulcerative colitis (6). CXCL5 is important in growth and development of colorectal cancer, implicating a future role in both cancer therapy and diagnosis (7). CXCL5 has also been associated with connective tissue remodeling (2). Protein DetailsPurity >95% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01EU/µg as determined by the LAL method Biological Activity The biological activity of Human ENA-78, 9-78 a.a. was determined by its ability to induce myeloperoxidase release from cytochalasin B treated human neutrophils (Schröder, J. et al., 1987, J. Immunol. 139:3474) or chemotaxis of mouse BaF/3 cells transfected with human CXCR-2. The expected ED<sub>50</sub> for these effects are typically 0.1 - 0.3 μg/ml or 0.15 - 0.75 ng/ml, respectively. Protein Accession No. Amino Acid Sequence agpa aavlrelrcv clqttqgvhp kmisnlqvfa igpqcskvev vaslkngkei cldpeapflk kviqkildgg nken N-terminal Sequence Analysis Ala37 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human CXCL5 is Mr 8.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 7 kDa. Predicted Molecular Mass 8.4 Formulation This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) containing BSA as a carrier protein pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Strieter, RM. et al. (2005) Cytokine Growth Factor Rev. 16:593 2. Persson, T. et al. (2003) Clin. Exp. Allergy 33:531 3. Dean, RA. et al. (2008) Blood 112:3455 4. Chang, MS. et al. (1994) J. Biol. Chem. 269:25277 5. Rajashekhar, G. et al. (2007) Physiol. Genomics 31:104 6. Wågsäter, D. et al. (2007) Internatl. J. Oncol. 31:97 7. Nagelkerke, F. et al. (2008) Clin. Cancer Res. 14:2276 Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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