Recombinant Human DAN

DAN is a secreted glycoprotein related to Xenopus laevis cerberus. It is being referred to also as DAND1 [DAN domain family member 1]. The gene has been described also as NO3 gene, which shows tumor suppressor activity in cells transformed by the src oncogene.¹ DAN belongs to a gene family, termed CAN family, that includes the head-inducing factor Gremlin, Cerberus, PRDC.² DAN and several other members of the can family act as antagonists of BMP and appear to block BMP signaling by preventing bone morphogenetic proteins from interacting with their receptors. DAN has recently been demonstrated to have a tumor-suppressive activity in vitro and may have an important role in regulation of the entry of cells into the S phase.³

Fc Fusion Protein

apppinklal fpdksawcea knitqivghs gceaksiqnr aclgqcfsys vpntfpqste slvhcdscmp aqsmweivtl ecpgheevpr vdklvekilh cscqacgkep sheglsvyvq gedgpgsqpg thphphphph pggqtpeped ppgaphteee gaedkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdeltknqv sltclvkgfy psdiavewes ngqpennykt tppvldsdgs fflyskltvd ksrwqqgnvf scsvmhealh nhytqkslsl spgk

Ala17

Lyophilized

The predicted molecular weight of Recombinant Human DAN is Mr 44.3 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 55-65 kDa.

44.3

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Ozaki, T. and Sakiyama, S. (1993) Proc. Natl Acad. Sci. USA 90:2593-2597
2. Rossant, J. et al. (1999) Dev Biol. 209(1):98-110.
3. Sakiyama S. et al. (1995) Biochem Biophys Res Commun. 216(1):209-15.