Recombinant Human EDAR

Ectodysplasin A receptor (EDAR), also known as ED1R, is a cell surface receptor of the tumor necrosis factor receptor (TNFR) family. Defects in EDAR are a cause of hypohidrotic ectodermal dysplasia (HED).¹ It is characterized by abnormalities in the hair, teeth and sweat glands. EDAR is also the genetic determinant of hair fiber thickness variation among Asian populations.² EDA has two splice variants, EDA-A1 and EDA-A2, which differ by only two amino acids. Despite this minor difference, EDAR only binds to EDA-A1, whereas EDA-A2 binds to XEDAR. It mediates the activation of NF-κB and JNK and may promote caspase independent cell death.³

Fc Fusion Protein

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Glu27

Lyophilized

The predicted molecular weight of Recombinant Human EDAR is Mr 44.3 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 55-60 kDa.

44.3

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Headon, DJ. et al. (2008) Hum. Genet. 29:1405
2. Tokunaga, K. et al. (2008) Hum. Genet. 124:179
3. Kumar, A. et al. (2001) J. Biol. Chem. 276:2668