Recombinant Human Eotaxin-2

Eotaxin-2 is a CC chemokine that is a specific agonist for the chemokine receptor CCR3. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 induces chemotaxis of eosinophils as well as basophils and the release of histamine and leukotriene C4 from basophils. Eotaxin-2 has the same efficacy as eotaxin, but is somewhat less potent. ¹ Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells.

The biological activity of Human Eotaxin-2 was determined by its ability to chemoattract hCCR-3 transfected rat myeloid Y3 cells and mCCR-3 transfected BaF/3 cells. The expected ED₅₀ for this effect is typically 0.01 - 0.05 μg/ml for both assays.

AAB51135.1

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Val27

Lyophilized

The predicted molecular weight of Recombinant Human Eotaxin-2 is Mr 10.6 kDa.

10.6

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Baggiolini, M. et al. (1997) J. Exp. Med. 185: 2171