Recombinant Human Eotaxin-3 (CCL-26)

Eotaxin-3/CCL-26 belongs to a family of CC chemokines, which are potent chemoattractants for eosinophils that signal through the CCR3 receptor. ¹ It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells and monocytes. It has been reported that eotaxin-3 is an antagonist for CCR2.² Eotaxin-3 has similar activity to Eotaxin and Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Eotaxin-3 is up-regulated in the context of a T helper 2 mediated inflammatory bowel disease via the signal transducer and activator of transcription 6, thus suggesting that the intestinal epithelium actively participates in the recruitment of eosinophils at the site of inflammation.¹

The biological activity of Human Eotaxin-3 was determined by its ability to induce chemotaxis of mouse BaF/3 cells stably expressing mCCR3. The expected ED₅₀ for this effect is typically 0.2 - 0.8 μg/ml.

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Thr24

Lyophilized

The predicted molecular weight of Recombinant Human Eotaxin-3 is Mr 8.4 kDa.

8.4

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Cuber, JC.et al. (2005) Int J Biochem Cell biol. 37: 2559
2. Gerber, B. et al. (2004) J Biol Chem. 279: 23357