Recombinant Human FGF-17
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BackgroundFibroblast growth factor 17, also known as FGF17 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-17 is most similar to FGF-8 and is highly conserved between mouse and human (93 % identity).1,2 FGF17 is required for several complex social behaviors and studies suggest that disturbances in FGF17 signaling may contribute to neuropsychiatric diseases that affect such behaviors.3 Protein DetailsPurity >95% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Human FGF-17 was determined by its ability to stimulate 3H-thymidine incorporation by NR6R-3T3 fibroblast. The expected ED<sub>50</sub> for this effect is typically 15 - 60 ng/ml, in the presence of 1 μg/ml heparin. Protein Accession No. Amino Acid Sequence tqgenhpspn fnqyvrdqga mtdqlsrrqi reyqlysrts gkhvqvtgrr isataedgnk faklivetdt fgsrvrikga esekyicmnk rgkligkpsg kskdcvftei vlennytafq narhegwfma ftrqgrprqa srsrqnqrea hfikrlyqgq lpfpnhaekq kqfefvgsap trrtkrtrrp qplt N-terminal Sequence Analysis Thr23 (with and without an N-terminal Met) State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human FGF-17 is Mr 22.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 21.5 and 22.6 kDa (reducing conditions). Predicted Molecular Mass 22.6 Formulation This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Hoshikawa, M. et al. (1998) Biochemical and Biophysical Research Communications 244: 187 2. Xu, J. et al. (1999) Mechanisms of Development 83: 165 3. Mucke, L. et al. (2008) Genes Brain Behav. 7: 344 Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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