Recombinant Human Follistatin

Follistatin (FST) is a glycosylated single-chain protein. It specifically inhibits the secretion of FSH from the pituitary.¹ Follistatin/activin-binding protein binds to heparan sulfate side chains of proteoglycans on the granulosa cell surface to regulate the various actions of activin.² Follistatin regulates a variety of biological functions, including cell proliferation, differentiation, and apoptosis. Follistatin plays a critical role in the production of multiple-organ metastasis, predominantly by inhibiting the angiogenesis.³

The biological activity of Human FS was determined by its ability to neutralize Activin-induced bioactivity on K562 cells (erythroid differentiation). The expected ED₅₀ for this effect is typically 0.1 - 0.4 μg/ml in the presence of 7.5 ng/ml of rhActivin A.

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Met

Lyophilized

The predicted molecular weight of Recombinant Human FS is Mr 31 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 40 kDa.

31

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Ling, N. et al. (1989) Mol Endocrinol. 3: 651
2. Sugino, H. et al. (1991) J Biol Chem. 266: 19432
3. Sone, S. et al. (2008) Clin Cancer Res. 14: 660