Recombinant Human HGF

Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, in adult organ regeneration and in wound healing.¹

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.

The biological activity of Human HGF was determined by measuring its ability to stimulate 3H-thymidine incorporation in the HGF responsive monkey epithelial cell line, 4MBr-5 (Rubin, J.S. et al., 1991, Proc. Natl. Acad. Sci. 88:415 - 419). The expected ED₅₀ for this effect is typically 20.0 - 40.0 ng/ml.

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alpha chain: No results obtained, Gln32 predicted; beta chain: Val495

Lyophilized

This protein consists of a disulfide-linked heterodimer and an unprocessed single chain. The predicted molecular mass is 53.7 kDa (α chain), and 26 kDa (β chain). The actual molecular mass of Recombinant Human HGF is Mr 60-70 kDa under nonreducing conditions; and 60 kDa and 33-34 kDa under reducing conditions.

This recombinant protein was lyophilized from a 0.2 μm filtered solution in sodium phosphate (NaH₂PO₄) and sodium chloride (NaCl).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Lyon, M. et al. (2000) Proteoglycans: structure, biology and molecular interactions.: 27