Recombinant Human HGF (NS0 Cell Expressed)

Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, in adult organ regeneration and in wound healing.¹

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.

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alpha chain: No results obtained; Gln32 predicted beta chain: Val495

Lyophilized

This protein consists of a disulfide-linked heterodimer. The predicted molecular mass is 53.7 kDa (α chain) and 26 kDa (β chain). The actual molecular weight of Recombinant Human HGF is Mr 65-75 kDa under nonreducing conditions; and 60-65 kDa and 30-40 kDa under reducing conditions.

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Lyon, M. et al. (2000) Proteoglycans: structure, biology and molecular interactions.: 27
2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.