Recombinant Human HGF R
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BackgroundHepatocyte growth factor receptor (HGFR), also known as c-Met, plays an important roles in angiogenesis and tumor growth.1 C-Met is a receptor tyrosine kinase expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC-γ. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis.2,3 Protein DetailsPurity >95% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Human HGF R was determined by its ability to bind human HGF. Immobilized rhHGF R/Fc at 1 μg/ml (100 μL/well) can bind rhHGF (0.1 - 5 ng/ml) in an ELISA type assay. Fusion Protein Tag Fc Fusion Protein Protein Accession No. Amino Acid Sequence eckeal aksemnvnmk yqlpnftaet piqnvilheh hiflgatnyi yvlneedlqk vaeyktgpvl ehpdcfpcqd csskanlsgg vwkdninmal vvdtyyddql iscgsvnrgt cqrhvfphnh tadiqsevhc ifspqieeps qcpdcvvsal gakvlssvkd rfinffvgnt inssyfpdhp lhsisvrrlk etkdgfmflt dqsyidvlpe frdsypikyv hafesnnfiy fltvqretld aqtfhtriir fcsinsglhs ymemplecil tekrkkr (α chain) stk kevfnilqaa yvskpgaqla rqigaslndd ilfgvfaqsk pdsaepmdrs amcafpikyv ndffnkivnk nnvrclqhfy gpnhehcfnr tllrnssgce arrdeyrtef ttalqrvdlf mgqfsevllt sistfikgdl tianlgtseg rfmqvvvsrs gpstphvnfl ldshpvspev ivehtlnqng ytlvitgkki tkiplnglgc rhfqscsqcl sappfvqcgw chdkcvrsee clsgtwtqqi clpaiykvfp nsapleggtr lticgwdfgf rrnnkfdlkk trvllgnesc tltlsestmn tlkctvgpam nkhfnmsiii snghgttqys tfsyvdpvit sispkygpma ggtlltltgn ylnsgnsrhi siggktctlk svsnsilecy tpaqtistef avklkidlan retsifsyre dpivyeihpt ksfisggsti tgvgknlnsv svprmvinvh eagrnftvac qhrsnseiic cttpslqqln lqlplktkaf fmldgilsky fdliyvhnpv fkpfekpvmi smgnenvlei kgndidpeav kgevlkvgnk scenihlhse avlctvpndl lklnselnie wkqaisstvl gkvivqpdqn ft(β chain)hiegrmdp kscdkthtcp pcpapellgg psvflfppkp kdtlmisrtp evtcvvvdvs hedpevkfnw yvdgvevhna ktkpreeqyn styrvvsvlt vlhqdwlngk eykckvsnka lpapiektis kakgqprepq vytlppsrde ltknqvsltc lvkgfypsdi avewesngqp ennykttppv ldsdgsffly skltvdksrw qqgnvfscsv mhealhnhyt qkslslspgk hhhhhh
N-terminal Sequence Analysis Glu25 & Ser308 State of Matter Lyophilized Predicted Molecular Mass This protein consists of two disulfide-linked heterodimers (proteolytically cleaved α and β subunits). The predicted molecular mass is 32.5 kDa (α chain) and 96.7 kDa (β chain). The actual molecular mass of Recombinant Human HGF R is Mr 43-50 kDa (α chain) and 120-125 kDa (β chain). Formulation This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with trehalose pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. McDonald, DM. et al. (2008) BMB Rep. 41: 833 2. Park, M. et al. (1998) Oncogene 16: 833 3. Comoglio, PM. et al. (1998) J Cell Sci. 111: 237 Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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