Recombinant Human IGFBP-2

Insulin-like growth factor binding protein 2, also known as IGFBP2, is an extracellular unglycosylated polypeptide that specifically binds IGF and modulates its effects.¹ IGFBP-2 has direct effects on breast cancer proliferation via stimulation of critical signaling pathways.²

The biological activity of Human IGFBP-2 was determined by its ability to inhibit the biological activity of rhIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The expected ED₅₀ for this effect is 0.03 - 0.09 μg/ml in the presence of 14 ng/ml rhIGF-II.

CAA34373

mevlfrcppc tperlaacgp ppvappaava avaggarmpc aelvrepgcg ccsvcarleg eacgvytprc gqglrcyphp gselplqalv mgegtcekrr daeygaspeq vadngddhse gglvenhvds tmnmlggggs agrkplksgm kelavfrekv teqhrqmgkg gkhhlgleep kklrpppart pcqqeldqvl eristmrlpd ergplehlys lhipncdkhg lynlkqckms lngqrgecwc vnpntgkliq gaptirgdpe chlfyneqqe acgvhtqrmq

Met

Lyophilized

The predicted molecular weight of Recombinant Human IGFBP-2 is Mr 31 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 36 kDa.

31

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Luthman, H. et al. (1991) Biochem Biophys Res Commun. 176: 1250
2. Disis, ML. et al. (2008) Cancer Res. 68: 8400