Recombinant Human IGFBP-5

Insulin-like growth factor binding protein 5, also known as IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. It is expressed highly in the kidney. IGFBP-5 has an autonomous role in the regulation of cell fate in development and in tumourigenesis.¹

The biological activity of Human IGFBP-5 was determined by by its ability to inhibit the biological activity of rhIGF-I or rhIGF-II on MCF-7 cells.² In the presence of 14 ng/mL rhIGF-II, the expected ED₅₀ for this effect is typically 0.5 - 1.5 μg/mL.

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Glu28 & Met-Leu21

Lyophilized

The predicted molecular weight of Recombinant Human IGFBP-5 is Mr 28-29 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 35-37 kDa.

28

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Pell, JM. et al. (2004) J of Cell Science 117: 1737
2. Karey, KP. et al. (1988) Cancer Research 48:4083.