Recombinant Human IgG

Immunoglobulin G (IgG) is a monomeric immunoglobulin synthesized and secreted by plasma B cells. It is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids (1). IgG antibodies are predominately involved in the secondary antibody response (the main antibody involved in primary response is IgM) (2). Pro-inflammatory cytokines particularly IL-4 and IL-2 have a crucial role in activation of the IgG antibody response. IgG is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in utero (3). In addition, residual IgG absorbed through the placenta provides the neonate with humoral immunity before its own immune system develops.

This protein is meant for use as a control.

Fc Fusion Protein

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Ile

Lyophilized

The predicted molecular weight of Recombinant Human IgG is Mr 26.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 30-35 kDa.

26.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Junqueira, LC. and Carneiro, J. (2003) Basic Histology. Tenth edition, McGraw Hill Companies
2. Meulenbroek, AJ. et al. (1996) Human IgG Subclasses: Useful diagnostic markers for immunocompetence (Sanquin)
3. Pitcher-Wilmott, RW. et al. (1980) Clin. Exp. Immunol. 41:303