Recombinant Human IL-1 RAcP

IL-1R accessory protein (IL-1RAcP) is a transmembrane protein that interacts with IL-1R and is required for IL-1 signal transduction. IL-1RAcP is an indispensible molecule in the IL-1 receptor signal transduction complex, necessary to link events on the plasma membrane level to downstream signaling pathways, allowing IL-1-dependent activation of transcription factors and gene expression.¹ IL-1R AcP represents a novel target for blocking IL-1 function in human disease.²

HEK293-derived human IL-1 RAcP/IL-1 R3 protein Ser21-Glu359, with a C-terminal 6-His tag

sercddwgld tmrqiqvfed eparikcplf ehflkfnyst ahsagltliw ywtrqdrdle epinfrlpen riskekdvlw frptllndtg nytcmlrntt ycskvafple vvqkdscfns pmklpvhkly ieygiqritc pnvdgyfpss vkptitwymg cykiqnfnnv ipegmnlsfl ialisnngny tcvvtypeng rtfhltrtlt vkvvgspkna vppvihspnd hvvyekepge ellipctvyf sflmdsrnev wwtidgkkpd ditidvtine sishsrtede trtqilsikk vtsedlkrsy vcharsakge vakaakvkqk vpaprytvei egrmdpkscd kthtcppcpa pellggpsvf lfppkpkdtl misrtpevtc vvvdvshedp evkfnwyvdg vevhnaktkp reeqynstyr vvsvltvlhq dwlngkeykc kvsnkalpap iektiskakg qprepqvytl ppsrdeltkn qvsltclvkg fypsdiavew esngqpenny kttppvldsd gsfflysklt vdksrwhhhh hh qqgnvfscsv mhealhnhyt qkslslspgk

Ser21

Lyophilized

The predicted molecular weight of Recombinant Human IL-1 RAcP is Mr 40 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 55-65kDa.

40

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Martin, MU. et al. (1997) J Biol Chem. 272: 7727
2. Labow, MA. et al. (1998) J Immunol. 161: 5614