Recombinant Human IL-12

IL-12 plays a role in resistance against pathogens via the differentiation of naive T cells into Th1 cells. It stimulates the growth and function of T cells, blocks formation of new blood vessels, and contributes to antimycobacterial immune response. In addition, it promotes the production of IFN-γ and TNF-α and reduces IL-4 mediated suppression of IFN-γ. Consequently, this enhances the immunostimulatory and immunomodulatory effects of IFN-γ. In addition, there appears to be a link between IL-2 and the signal transduction of IL-12 in NK cells, which enhances the functional response of IL-12 via IFN-γ production and killing of target cells. Furthermore, IL-12 is thought to be associated with autoimmunity. IL-12 was shown to worsen the condition when administered to people already suffering from autoimmune diseases. Comparatively, inhibition of IL-12 (either through IL-12 gene knock-out in mice or treatment of mice with IL-12 mAbs) improved the disease.

The biological activity of Human Interleukin-12 is determined by the stimulation of IFN-gamma from NK cells co-stimulated with IL-18. The expected ED₅₀ for this effect is 1 x 10⁶ units/mg.

P29460 & P29459

( Human IL-12 p40) iwelkkdvyv veldwypdap gemvvltcdt peedgitwtl dqssevlgsg ktltiqvkef gdagqytchk ggevlshsll llhkkedgiw stdilkdqke pknktflrce aknysgrftc wwlttistdl tfsvkssrgs sdpqgvtcga atlsaervrg dnkeyeysve cqedsacpaa eeslpievmv davhklkyen ytssffirdi ikpdppknlq lkplknsrqv evsweypdtw stphsyfslt fcvqvqgksk rekkdrvftd ktsatvicrk nasisvraqd ryyssswsew asvpcs, (Human IL-12 p35) nlpvatpdpg mfpclhhsqn llravsnmlq karqtlefyp ctseeidhed itkdktstve aclpleltkn esclnsrets fitngsclas rktsfmmalc lssiyedlkm yqvefktmna kllmdpkrqi fldqnmlavi delmqalnfn setvpqkssl eepdfyktki klcillhafr iravtidrvm sylnas

Ile23 (p40) & Arg23 (p35)

Lyophilized

The predicted molecular weight of Recombinant Human IL-12 is Mr 70 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 41 kDa (p40) and 29 kDa (p35) (reducing conditions) and 60 kDa (non-reducing conditions).

70

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Kapsenberg, ML. et al. (1997) J. Immunol. 159: 28
2. Ritz, J. et al. (2001) Blood. 97(12):3860-6.
3. Barnes, PF. et al. (1994) J Clin Invest. 93(4):1733-9.