Recombinant Human IL-17

Interleukin 17 (IL-17) is a T cell cytokine that induces monocyte migration and inflammation.¹ IL-17-producing cell could serve as a potential prognostic marker and a novel therapeutic target for hepatocellular carcinoma (HCC).² IL-17 may be important in recruiting monocytes into the joints of patients with RA, supporting IL-17 as a therapeutic target in RA.¹

The biological activity of Human IL-17A was determined by its ability to induce IL-6 production by NHDF cells. The expected ED₅₀ for this effect is typically 2 - 6 ng/mL.

The DNA sequence encoding the 136 amino acid residue mature human IL-17 protein (Ile 20 - Ala 155) was inserted into a suitable expression vector and was expressed in E. coli (Yao, Z. et al. 1995, J. Immunol. 155:5483 - 5486).

Lyophilized

The predicted molecular weight of Recombinant Human IL-17A is Mr 16 kDa.

16

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Pope, RM. et al. (2009) J Immunol. 182: 3884
2. Zheng, L. et al. (2009) J Hepatol. 50(5):980-989.