Recombinant Human IL-17 R

Interleukin-17 receptor (IL-17R) Type 1 transmembrane glycoprotein which binds interleukin 17. IL-17R signaling is critical for pulmonary neutrophil recruitment and host defense against Gram-negative bacteria.¹ It represents a marker for the metastasizing ability of osteosarcoma.¹

The biological activity of Human IL-17R was determined by its ability to inhibit IL-17-induced IL-6 production by NHDF cells. The ED⁵⁰ for this effect is typically 0.03 - 0.1 μg/ml in the presence of 10 ng/ml rhIL-17.

The DNA sequence encoding the human IL-17 R extracellular domain protein sequence (Yao, Z., et al., (1997) Cytokine 9(11):794 - 800) was fused to the Fc region of human IgG1 via a polypeptide linked, inserted into a suitable mammalian expression vector and expressed in NSO cells.

Leu33

Lyophilized

The predicted molecular weight of Recombinant Human IL-17R is Mr 60 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 100 kDa.

60

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Facchini, A. et al. (2007) J clin. Research clin. oncol. 133: 1017