Recombinant Human IL-19

Interleukin-19 (IL-19) is a secreted protein that belongs to the IL-10 family of cytokines along with several other interleukins including IL-10, IL-20, IL-22, IL-24, IL-26, and several virus-encoded cytokines. Members of this family share partial homology in their amino acid sequences but they are dissimilar in their biological functions. IL-19 is expressed in resting monocytes and B cells and is up-regulated in monocytes following stimulation with granulocyte-macrophage colony-stimulating factor (GM-CSF) or lipopolysaccharide.¹ In addition, IL-19 may play important roles in inflammatory responses due to its ability to upregulate IL-6 and TNF-alpha as well as induce apoptosis.² Human and murine IL-19 share 71% amino acid sequence identity.

The biological activity of Human IL-19 was determined by its ability to induce proliferation of mouse BaF/3 cells co-transfected with human IL-20 Rα and IL-20 Rβ. The expected ED₅₀ for this effect is typically 0.5 - 1.5 ng/mL.

mlrrcli stdmhhiees fqeikraiqa kdtfpnvtil stletlqiik pldvccvtkn llafyvdrvf kdhqepnpki lrkissians flymqktlrq cqeqrqchcr qeatnatrvi hdnydqlevh aaaikslgel dvflawinkn hevmssa

Met

Lyophilized

The predicted molecular weight of Recombinant Human IL-19 is Mr 18 kDa.

18

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Chang, C. et al. (2003) J. of Biol. Chem. 278:3308
2. Chang, MS. et al. (2002) J. Immunol. 169:4288